Adsorption of phosphate over a novel magnesium-loaded sludge-based biochar.

The production of sludge-based biochar to recover phosphorus (P) from wastewater and reuse the recovered phosphorus as agricultural fertilizer is a preferred process. This article mainly studied the removal of phosphate (PO4-P) from aqueous solution by synthesizing sludge-based biochar (MgSBC-0.1) from anaerobic fermentation sludge treated with magnesium (Mg)-loading-modification, and compared it with unmodified sludge-based biochar (SBC). The physicochemical properties, adsorption efficiency, and adsorption mechanism of MgSBC-0.1 were studied. The results showed that the surface area of MgSBC-0.1 synthesized increased by 5.57 times. The material surface contained MgO, Mg(OH)2, and CaO nanoparticles. MgSBC-0.1 can effectively remove phosphate in the initial solution pH range of 3.00-7.00, with a fitted maximum phosphorus adsorption capacity of 379.52 mg·g-1. The adsorption conforms to the pseudo second-order kinetics model and Langmuir isotherm adsorption curve. The characterization of the adsorbed composite material revealed the contribution of phosphorus crystal deposition and electrostatic attraction to phosphorus absorption.

Multi-omics analysis reveals the collaboration and metabolisms of the anammox consortia driven by soluble/non-soluble Fe(III) as the sole iron element.

Iron is recognized as a physiological requirement for anammox bacteria (AnAOB), with Fe(II) considered to be the most effective form. However, Fe(III), instead of Fe(II) is the common iron form in natural and artificial ecosystems. In this study, the nitrogen removal performance and metabolic mechanisms in anammox consortia with soluble and non-soluble Fe(III) as the sole iron element were investigated. After the 150-day operation, the soluble (FeCl3) and insoluble (Fe2O3) Fe(III)-fed anammox systems reached nitrogen removal rates of 71.84 ± 0.80% and 50.20 ± 0.98%, respectively. AnAOB could survive with soluble (FeCl3) or insoluble (Fe2O3) Fe(III) as the sole iron element, reaching relative abundances of 18.49% and 13.16%, respectively. The results show that the formation of anammox core consortia can enable AnAOB's survival to adverse external conditions of Fe(II) deficiency. Metagenomic and metatranscriptomic analysis reveal that Ca. Kuenenia can only uptake Fe(II) into the cell for metabolisms either independently through the extracellular electron transfer or with the cross-feeding of symbiotic microbes. This study provides insight into the utilization and metabolic mechanisms of Fe(III) in Ca. Kuenenia-dominated consortia, and deepens the understanding of anammox core consortia in the nitrogen, carbon, and iron cycling, further promoting the practical applications of anammox processes.

Efficiency and mechanism of phosphate adsorption and desorption of a novel Mg-loaded biochar material.

Phosphate removal is complicated by the need for resource recovery. Biochar shows promise for efficient phosphate adsorption, but it must be modified to enhance its adsorption capacity. In this work, magnesium (Mg)-loaded biochar was synthesized through a two-step dipping and calcination process, and the MgBC600 product was used to adsorb phosphate from simulated water and biogas slurry wastewater. The phosphate adsorption capacity of Mg-loaded biochar was 109.35 mg/g, which was 12 times higher than that of unmodified biochar. The R2 of the Langmuir and pseudo-second-order kinetic models were 0.988 and 0.990, respectively, which fitted the phosphate adsorption process of MgBC600. Phosphate adsorption by MgBC600 was a spontaneous and endothermic process. The adsorption mechanism study showed that phosphate adsorption was controlled by the formation and electrostatic attraction of MgHPO4. In addition, 98% of chemically adsorbed phosphate was released after regeneration. Using phosphate-adsorbed MgBC600 as a soil amendment, Arabidopsis thaliana was 1.47 times higher than that in the biochar-only group, demonstrating that this is a promising strategy for enhancing phosphate adsorption efficiency and adsorbent recycling.

Insight into the mechanism of nutrients removal and response regulation of denitrifying phosphorus removal system under calcium ion stress.

The influent quality is an important factor affecting the nutrients removal and operational stability of denitrifying phosphorus removal (DPR) system. This study investigated the effects of calcium ion (Ca2+) on the nutrients removal, nitrogen oxide (N2O) release, microbial community, and quorum sensing in DPR system. Results showed that high accumulation of Ca2+ had a significant impact on the carbon footprint of DPR system. Specifically, N2O release reached 2.11 mg/L under Ca2+ of 150 mg/L, which represented 214.93% increase compared to 0 mg/L of Ca2+. The DPR system demonstrated its adaptability to elevated Ca2+ concentrations by modifying key enzyme activities involved in nitrogen and phosphorus removal, altering the microbial community structure, and adjusting the type and content of signal molecules. These findings hold significant implications for understanding the stress mechanism of Ca2+ on DPR system, ultimately aiding in the maintenance and enhancement of stable operational performance in biological wastewater treatment process.

Aquatic plant root exudates: A source of disinfection byproduct precursors in constructed wetlands.

Aquatic plant-derived dissolved organic matter (DOM) in water bodies is an important source of disinfection byproduct (DBP) precursors. It is therefore very important to investigate DBP formation, and the main DBP precursors that enter drinking water during treatment processes. In this study, Lythrum salicaria root extract (LSRE) and Acorus calamus root extract (ACRE) were analyzed. The LSRE and ACRE were chlorinated and disinfected to generate trihalomethanes, haloacetic acids, haloketones, and haloacetaldehydes. The DBP formation potential of LSRE, dominated by humus, was higher than that of Suwannee River natural organic matter (SRNOM), and trichloroacetic acid was the main DBP. It was calculated that 2.09 % of the increased DOC brought by the surface flow wetland planted with emergent aquatic plants, and the contribution rates of TCMFP, DCAAFP and TCAAFP in effluent were 3.34 %, 3.23 % and 3.05 %, respectively. A total of 706 chlorinated-formula were detected by FTICR-MS, among which mono- and di-chlorinated formulae were the most abundant. Macromolecular hydrophobic organics and tannins were the main precursors for LSRE. Unlike LSRE, the DOM composition of ACRE was dominated by protein or aliphatic compounds; therefore, the risk of DBP formation was not as high as that for LSRE. This study is the first to determine the risk of DBP formation associated with aquatic plant root extracts, and confirmed that tannins in plant-derived DOM are more important DBP precursors than lignins.

Process model for flow-electrode capacitive deionization for energy consumption estimation and system optimization.

Flow-electrode capacitive deionization (FCDI) is a new technology for ion removal that delivers sustainable deionization performance. However, FCDI consumes relatively high amounts of energy compared with other conventional desalination technologies, which hinders the industrial application of FCDI. In this study, the energy consumption of each FCDI component was simulated using a steady-state FCDI model to investigate and optimize the main components of energy consumption. Overall, the established process model can be used for theoretical investigation and enhancing our fundamental understanding of the energy consumption of each FCDI component, and provides the design and optimization of FCDI systems. The results showed that the energy consumption of the flow electrodes dominated under most conditions. Changing the operating parameters could obviously affect energy consumption and the energy consumption structure. However, increasing the flow rate and activated carbon (AC) content of the flow-electrode could decrease the energy consumption of the electrode, and the energy consumed by the ion-exchange membranes (IEMs) and desalination chamber was the greatest. These two parts of energy consumption could not be significantly reduced by changing operational parameters. Thus, to further reduce the energy consumption, optimization of the FCDI equipment was carried out by adding titanium mesh to the flow electrodes and the desalination chamber of the FCDI cell. The results showed that the energy consumption of optimized FCDI decreased by 51.9% compared with the original FCDI. The long-term experiment using optimized FCDI showed good stability and repeatability.

Deciphering the effects of antibiotics on nitrogen removal and bacterial communities of autotrophic denitrification systems in a three-dimensional biofilm electrode reactor.

In this study, three-dimensional biofilm electrode reactors (3D-BERs) were constructed, and the effects of metronidazole (MNZ) on the nitrogen removal performance and bacterial communities of autotrophic denitrification systems were evaluated. The results showed that nitrogen removal decreased slightly as the MNZ concentration increased. Specifically, nitrate-nitrogen removal efficiency decreased from 97.98% to 89.39%, 86.93%, 82.64%, and 82.77% within 12 h after the addition of 1, 3, 5, and 10 mg/L MNZ, respectively. The 3D-BERs showed excellent MNZ degradation ability, especially at a concentration of 10 mg/L. The MNZ removal efficiency could be as high as 94.38% within 6 h, and the average removal rate increased as the MNZ concentration increased. High-throughput sequencing results showed significant changes in the bacterial community under different MNZ concentrations. As the antibiotic concentration increased, the relative abundances of Hydrogenophaga and Silanimonas increased, from only 0.09% and 0.01% without antibiotics to 3.55% and 2.35%, respectively, at an antibiotic concentration of 10 mg/L. Changes in antibiotic concentration altered the abundances of genes involved in nitrogen metabolism. Redundancy analysis showed that MNZ removal efficiency was positively correlated with SBR1031, SC-I-84, Hydrogenophaga, Silanimonas and Denitratesoma, whereas the removal efficiencies of nitrate-nitrogen and total nitrogen were negatively correlated with these genera. The results of this study provide a theoretical basis for studying the toxic effects of antibiotics on the denitrification process and also provide guidance for the control of antibiotics and nitrogen pollution in ecosystems.

Light-dependent enhancement of sulfadiazine detoxification and mineralization by non-photosynthetic methanotrophs.

Co-metabolism and photodegradation are two approaches for remediating trace organic compounds (TOrCs), however, interactions between the two with regards to TOrCs degradation have not been elucidated. In this study, sulfadiazine (SDZ) was chosen as a representative TOrC and Methylocystis bryophila as a typical strain. Under light conditions, about 80.6% of SDZ was removed by M. bryophila, but only 7.6% or 28.9% of SDZ was eliminated by either individual photodegradation or by co-metabolism. The SDZ stimulated more extracellular organic matter (EOM) production by M. bryophila. The enhanced SDZ degradation was attributed to indirect photolysis caused by the excited triplet state of EOM (3EOM*) and co-metabolism. The UPLC-QTOF-MS analysis showed that due to co-metabolism, the pyrimidine ring was broken and could further be oxidized into smaller molecules under light conditions, such as formic and acetic acids. The SDZ mineralization ratio increased from 9.9% under the co-metabolic condition alone to 36.5% under co-metabolism coupled with photodegradation. The Ames tests confirmed that the SDZ degradation products by co-metabolism were mutagenic, however, their toxicity was ameliorated by light during co-metabolism. In conclusion, light plays a crucial role in co-metabolic processes of TOrCs.

Influence of low air pressure on combined nitritation and anaerobic ammonium oxidation process.

At high altitude, wastewater aeration efficiency is low, which is detrimental to nitrification in conventional biological nitrogen removal. The combined partial nitritation and anaerobic ammonium oxidation (CPNA) process requires little oxygen and can be appropriate in low-pressure conditions. As such, in this study, we investigated the effect of air pressure on CPNA using a laboratory-scale reactor. We found that low air pressure promoted the removal of total inorganic nitrogen (TIN), achieving a TIN removal rate of 43,000 mg·N/(kg·VSS·d). The secretion of extracellular polymeric substances under low air pressure was not significantly different from that under ordinary air pressure, indicating no adverse effects on microbial aggregation ability, stability, or settleability. The abundance of aerobic ammonia-oxidizing bacteria (AeAOB) increased from 0.2% to 5.6%, and the activity of anaerobic ammonia-oxidizing bacteria (AnAOB) enhanced, giving AeAOB and AnAOB a competitive advantage over nitrite-oxidizing bacteria, thus forming a microbial community structure favorable to the CPNA process. Our further analysis of the results of batch tests in serum bottles confirmed the positive effect of low air pressure on the anaerobic ammonium oxidation (anammox) process, with a 28.5% ± 1.9% improvement in the specific anammox rate at 70 kPa compared with 100 kPa. AnAOB activity increased, which was reflected in the intracellular heme content increasing from 0.56 ± 0.18 µmol/(g·VSS) at 100 kPa to 2.56 ± 0.20 µmol/(g·VSS) at 70 kPa. We clarified the CPNA-process-promoting effect of low air pressure, which shows potential for nitrogen removal in high-altitude regions.

Visible-Light-Driven Ag-Doped BiOBr Nanoplates with an Enhanced Photocatalytic Performance for the Degradation of Bisphenol A.

Based on the low utilization rate of visible light and the high-charge carriers-recombination efficiency of bismuth oxybromide (BiOBr), in this work, noble metal Ag was used to modify BiOBr, and Ag-doped BiOBr nanoplates (Ag-BiOBr) were obtained through a one-step hydrothermal method. Compared with BiOBr, the absorption edge of Ag-BiOBr showed a redshift from 453 nm to 510 nm, and the absorption efficiency of visible light was, obviously, improved. Bisphenol A (BPA) was chosen as the target pollutant, to evaluate the photocatalytic performance of the samples. Ag0.1-BiOBr showed the highest degradation efficiency. The intrinsic photocatalytic activity of Ag0.1-BiOBr, under visible light, was approximately twice as high as that of BiOBr. In this way, a new visible-light-driven photocatalyst was proposed, to fight against organic pollution, which provides a promising strategy for water and wastewater treatment.

Electron-Level Mechanistic Insights into Ce Doping for Enhanced Efficiency Degradation of Bisphenol A under Visible Light Irradiation.

Bismuth oxybromide (BiOBr), with its special layered structure, is known to have potential as a visible-light-driven photocatalyst. However, the rapid recombination and short lifetime of the photogenerated carriers of BiOBr restrict its photocatalytic efficiency for the degradation of organic pollutants. Given the similar ionic size of Ce and Bi, Ce atoms might be easily introduced into the crystal of BiOBr to tailor its band structure. In this study, Ce doped BiOBr (Ce-BiOBr) samples with different percentages of Ce contents were prepared via a hydrothermal method. The intrinsic photocatalytic efficiency of Ce0.2-BiOBr for the degradation of bisphenol A (BPA) was 3.66 times higher than that of pristine BiOBr under visible light irradiation. The mechanism of Ce-doping modification for the enhanced photocatalytic performance was demonstrated based on a series of experiments and DFT calculation. The narrowed bandgap, the enhanced charge separation efficiency and Ce-doping energy level contributed to the remarkable photocatalytic performance of Ce-BiOBr.

Mixotrophic Cultivation of Microalgae Using Biogas as the Substrate.

Biogas utilization through biotechnology represents a potential and novel technology. We propose the microalgal mixotrophic cultivation to convert biogas to microalgae-based biodiesel, in which methanotroph was co-cultured to convert CH4 to organic intermediate (and CO2) for microalgal mixotrophic growth. This study constructed a co-culture of Methylocystis bryophila (methanotroph) and Scenedesmus obliquus (microalgae) with biogas feeding. Compared with the single culture of S. obliquus, higher microalgal biomass but a lower chlorophyll concentration was observed. The organic metabolism-related genes were upregulated, verifying microalgal mixotrophic growth. The stoichiometric calculation of M. bryophila culture shows that M. bryophila tends to release organic matter rather than grow under a low O2 content. M. bryophila rarely grew under five different light intensities, indicating that M. bryophila acts as a biocatalyst in the co-culture. The organic intermediate released by methanotroph increased the maximum biomass of microalgal culture, accelerated nitrogen absorption, accumulated more monounsaturated fatty acids, and improved the adaptation to light. The co-culture of microalgae and methanotroph may provide new opportunities for microalgae-based biodiesel production using biogas as a substrate.

Simultaneous denitrification and antibiotic degradation of low-C/N-ratio wastewater by a three-dimensional biofilm-electrode reactor: Performance and microbial response.

Three-dimensional biofilm-electrode reactors (3D-BERs) were fabricated and used to simultaneously remove nitrate and metronidazole (MNZ) from low-C/N-ratio wastewater. The results showed that 1 mg/L MNZ significantly promoted nitrate removal. After MNZ was added to the reactor, the removal efficiencies of total nitrogen (TN) and NO3--N increased significantly from 18.97% and 52.09% to 71.63% and 99.98% within 6 h, respectively. The MNZ-removal kinetics conformed to a pseudo-first-order model, and the removal rate constant reached a maximum value of 0.853 h-1, which was 4.1 and 2.8 times higher than that of pure microorganisms and pure electrochemical reactors, respectively. This indicated that the 3D-BERs constructed in this study were capable of simultaneous MNZ degradation and denitrification. In the presence of nitrate, six MNZ-degradation intermediates were identified, and four MNZ transformation pathways were proposed, including cleavage of hydroxyethyl groups, reduction of nitro groups, N-denitration, and deprotonation of side-chain hydroxyl groups. High-throughput sequencing revealed that the reactor was rich in various MNZ-degraders and denitrifiers, such as Hydrogenophaga, Methylomonas, Crenohrix, Dechloromonas, and Methylophilus. A function prediction analysis of nitrogen metabolism showed that the 3D-BER reactor with MNZ had higher denitrification activity than the other reactors tested. It was speculated that the intermediates produced by MNZ could act as carbon sources allowing denitrifying bacteria to perform denitrification, which made a nonnegligible contribution to the removal of nitrogen.

In-situ monitoring of membrane fouling migration and compression mechanism with improved ultraviolet technique in membrane bioreactors.

An improved UV spectrum in-situ monitoring system was applied to explore the membrane fouling behavior in membrane bioreactors (MBRs). The changes in absorbance curve illustrated that the formation of a stubborn fouling layer includes the migration and compression of membrane surface foulants. The initial flux negatively correlates with the migration degree (unevenness) of membrane fouling, while fiber length is positively correlated. In further experiments, ultrasonic thickness measurement excludes fouling layer compression caused by spatial collapse under external force. Moisture content measurement tests demonstrated that the moisture content changed from 52% to 31% after fouling layer compression, which confirmed that the fouling layer compression is mainly caused by the "high pressure dehydration effect". Finally, a membrane backwashing strategy based on fouling layer compression theory indicated that the backwashing process should be carried out at a stage where the accumulation of membrane fouling is constant but the fouling layer is not compressed.

Modelling of self-sustainable microbial fuel cell type oil sensors based on restricted oxygen transfer and two-population competition.

Oil leaks during oil industrial chain pose threats to the ecosystem. The microbial fuel cell-type oil sensor has been developed for early warning of such issues. Oil contacting with the sensor restricts oxygen availability and triggers correlative signal anomaly which serves as indicative of the oil presence. To extend its application for the real world, modelling of the sensor is required to pre-describe the signal behavior under unknown conditions. Therefore, by integrating Butler-Volmer, restricted oxygen transfer (ROT) and Monod equations, a dynamic ROT-MFC model with sufficient substrate precondition was developed. The ROT-MFC model was trained on the experimental single-oil-shock test (R 2 = 0.996) and validated by the experimental sequential-shocktest (R 2 = 0.998). Numerical analysis of the trained ROT-MFC model indicates that the single-shock detection has higher sensitivity (≥40.6 mV/detection) and the sequential-shocks detection spends a shorter response time (≤2.2 h). Besides, the sequential-shocks detection with proper strategy is more applicable due to flexible options on detection limit and working range. The model was further evolved into the TPC-ROT-MFC model by introducing a two-population competition (TPC) theory to describe performance under limited substrate conditions. Results indicate a critical substrate concentration range (42.1 to 62.8 mg-COD/L) for dividing baseline steadiness, and that the impact of substrate concentration on anodic charge transfer coefficient soars when the substrate concentration lessens furtherly. This sensor model is relatively easy to implement and may enhance practical use for design and operation.

Inexact left-hand side two-stage chance-constrained programming for booster optimization in water distribution system.

In water distribution system (WDS), chlorine is often injected as disinfectant to control the growth of microorganism in WDS. However, the chlorine reacts with organism to form disinfectant byproduct, which can bring risk to human health. As such, the chlorine at nodes in WDS should be kept between acceptable range, which is simulated based on the response at nodes corresponding to unit injection mass at boosters. To deal with the uncertainty in chlorine decay process and lower and upper chlorine concentration limits, an inexact left-hand-side chance-constrained programming (ILCCP) model was proposed in this paper and applied to two WDSs. The response coefficients matrix was expressed as random variables with normal probability distribution in the constraints of lower and upper limits, which was obtained through Monte Carlo simulation by linking with EPANET software. The intervals of injection mass were obtained by solving the ILCCP model with a two-step algorithm. Moreover, the effects of random bulk decay coefficients and interval of chlorine limits on the injection mass were analyzed and compared. The results indicated that the lower bounds of optimal injection mass increased with the rise of probability lever for lower limits, while the upper bounds decreased with the rise of the probability level for upper limits. The results can help managers determine the chlorine injection mass under uncertain scenarios, and can be applied to more complicated WDS to obtain meaningful results.

Simultaneous removal of sulfamethoxazole and enhanced denitrification process from simulated municipal wastewater by a novel 3D-BER system.

In this study, at an electric current intensity at 60 mA, more than 90.50 ± 4.76% of Sulfamethoxazole (SMX) was degraded. The strengthening of bacterial metabolisms and the sustainment of electrical stimulation contributed to the rapid removal of SMX and nitrates from simulated wastewater by a novel 3D-BER system. From the literature, very few studies have been performed to investigate the high risk of nitrates and antibiotics SMX found in wastewater treatment. The highest antibiotic SMX and nitrogen removal efficiency was 96.45 ± 2.4% (nitrate-N), 99.5 ± 1.5% (nitrite-N), 88.45 ± 1.4% (ammonia-N), 78.6 ± 1.0% (total nitrogen), and SMX (90.50 ± 4.76%), respectively. These results were significantly higher as compared to control system (p < 0.05). The highest denitrification efficiency was achieved at the pH level of 7.0 ± 0.20 - 7.5 ± 0.31. Lower or higher pH value can effect on an approach of heterotrophic-autotrophic denitrification. Moreover, low current intensity did not show any significant effect on the degradation, however, enhanced the removal rate of nitrate or nitrite as well as antibiotic SMX. Based on the results of HPLC and LC-MS/MS analysis, the intermediate products were proposed after efficient biodegradation of SMX. Finally, these results is expected to provide some new insights towards the high electric currents, changes the bacterial community structure, and the activated sludge which played an important role in the biodegradation of SMX and nitrates removal more efficiently.

Constraining nitrification by intermittent aeration to achieve methane-driven ammonia recovery of the mainstream anaerobic effluent.

Mainstream anaerobic treatment has the potential to capture organic energy, and represents a sustainable development trend, but with the problems of low biogas quality and dissolved methane emissions. In this study, methane-driven ammonia recovery of anaerobic effluent was proposed. A 380-day long-term experiment, which was divided into four phases according to different aeration modes, was conducted. The ammonia conversion and microbial characteristics shows that ammonia oxidizing bacteria (AOB) were constrained during Phases 2 (DO: <0.2 mg L-1) and 4 (DO: 0.1-1.6 mg L-1), and were active during Phase 3 (DO: 2-4 mg L-1). During phase 4, when the intermittent aeration was used, the total nitrogen removal rate was higher than during Phases 2 and 3, and nearly 100% ammonia was removed. Methylomonas, a genus of methane oxidizing bacteria (MOB), was enriched during Phase 4. The serum bottle experiment confirmed that the ammonia removal occurred through the MOB assimilation. The protein content in the CH4-added group was 35.5%, which was higher than in the group without CH4 (23.3%). The powerful ammonia assimilation and protein synthesis capabilities of MOB give a meaning to the anaerobic effluent for ammonia recovery and protein production. Intermittent aeration could be used to constrain AOB and improve ammonia recovery efficiency.

On-line monitoring of minor oil spills in natural waters using sediment microbial fuel cell sensors equipped with vertical floating cathodes.

Oil spills near natural water bodies pose considerable threats to aquatic ecosystem and drinking water system. Various detection techniques have been developed to identify the oil pollution in natural waters. These techniques mainly focus on large and major oil spills involving significant changes in environmental characteristics. However, monitoring of minor oil spills (from seepage and dripping) in waters remains a bottleneck, allowing inconspicuous and persistent oil contamination. To overcome this drawback, a sediment microbial fuel cell (SMFC) sensor equipped with a vertical floating cathode is developed for on-line and in-situ monitoring of minor oil spills in natural waters. The vertical floating cathode was intended for recognizing oil on water surface. Oil on the cathode will trigger current drop. Two kinds of natural sediments were adopted in two sensors (SMFC1 from a lake and SMFC2 from an urban stream) for comparison. Both showed linear relationship between net steady-state current decrease and oil dose (30.78 and 27.29 µA/mL of sensitivity, respectively). The current change process was fitted well to a pseudo-first order kinetic equation. A one-point/two-point dynamic identification methods were derived from the kinetic equation. Therefore, the detection time was shortened from 10 h to 10/30 min. The triggered current decrease was mainly attributed to the increase in internal resistance related to charge and mass transfer. Despite the power loss after oil contamination, results implied SMFC sensor could still achieve self-sustainability. This study shows that the SMFC sensor with vertical floating cathodes is applicable to monitoring the unnoticeable minor oil pollutions in natural waters.

Long-term electricity generation and denitrification performance of MFCs with different exchange membranes and electrode materials.

Different biocathode electrode materials (graphite felt and carbon brush, GF and CB) and exchange membranes (proton exchange membrane and cation exchange membrane, PEM and CEM) were used in three microbial fuel cell (MFC) configurations operated for 300-days to investigate the power generation and the COD and N removal performance. Results showed no effect on the COD removal (all above 96%); however, the power generation (46.11 mW·h) and denitrification performance (68.0 ± 1.6%) of the MFC-B (GF + PEM) system were higher than those of the other systems (MFC-A: CB + PEM; MFC-C: CB + CEM) (P < 0.01), and the power generation and denitrification performance of all three systems decreased with time (P < 0.01). By analyzing the physicochemical properties of the exchange membrane and cathode electrode materials, the reasons that affect the power generation performance of the system were clarified. Furthermore, the increase in bioelectricity enhanced the electricity-related nitrification and denitrification reactions. The average 300-day unit denitrification cost of MFC-A was 4.2 and 6.3 times that of MFC-B and MFC-C, respectively. Comprehensive consideration of electricity generation, denitrification, and service life, combined with cost analysis and better selection of construction materials, provides a theoretical basis for the long-term stable operation and sustainable application of MFCs.